Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Medchemexpress LLC TAE Powder / TAE Buffer | 100 POUCHES
TAE (Tris-acetate-EDTA) buffer is widely used in biological experiments, especially in agarose gel electrophoresis of nucleic acid. Linear dsDNA migrates quickly in electrophoresis, and DNA fragments (> 13 kb) can be separated effectively. TAE buffer is also suitable for DNA gel extraction.
- Consists of Tris-acetate and EDTA-2Na.
- Each pouch can be diluted to 1 L of 1x TAE buffer.
- Concentration of Tris-acetate is 40 mM and EDTA-2Na is 1 mM when diluted to 1 L of 1x TAE buffer.
- Provides a convenient way to make TAE solution.
- Eliminates the need to weigh and mix individual components.
- pH of the 1x TAE buffer is 8.3 ± 0.1 @ 25°C.
Medchemexpress LLC PBS-T Powder | 100 POUCHES
MCE PBS-T Powder (1 L of 1x) is a formulation consisting of NaCl, KCl, Phosphate, and Tween 20. It is extensively utilized as a washing buffer in immunoassay experiments such as ELISA and Western Blot. Additionally, it can be employed for antibody dilution and the preparation of blocking solutions. The product is conveniently packaged, with 20 pouches defined as the base specification, and larger sizes offered as incremental volumes of this base. Each pouch is designed to be diluted to 1 L of 1x PBS-T buffer.
- Widely used in ELISA and Western Blot.
- Can be used for antibody dilution and preparation of blocking solution.
- Consists of NaCl, KCl, Phosphate, and Tween 20.
- Each pouch dilutes to 1 L of 1x PBS-T buffer.
- Eliminates the need to weigh and mix individual components.
- Convenient way to make PBS-T buffer.
- Final 1x PBS-T buffer has a pH of 7.4 ± 0.2 @ 25°C.
Sigma Aldrich Fine Chemicals Biosciences HEPES hemisodium salt >=99% (titration) | 103404-87-1 | 100G
HEPES hemisodium salt >=99% (titration) | Purity: >=99% (titration) | Mol Wt: 249.3 | 103404-87-1 | 100G
AG Scientific Inc PBS Tablets 100 Tablets
Phosphate Buffered Saline is a buffer solution commonly used in biological research. PBS has many uses because it is isotonic and non-toxic to most cells. These uses include substance dilution and cell container rinsing.
Sigma Aldrich Fine Chemicals Biosciences HEPES buffer solution 1 M in H2O | 7365-45-9 | MFCD00006158 | 100ml
HEPES buffer solution 1 M in H2O | Mol Wt: | 7365-45-9 | MFCD00006158 | 100ml
Sigma Aldrich Fine Chemicals Biosciences Ethylenediaminetetraacetic acid >=98.0% (KT) | 60-00-4 | MFCD00003541 | 50G
Ethylenediaminetetraacetic acid >=98.0% (KT) | Purity: >=98.0% (KT) | Mol Wt: 292.24 | 60-00-4 | MFCD00003541 | 50G
Sigma Aldrich Fine Chemicals Biosciences MOBS >=99% (titration) | 115724-21-5 | MFCD00674916 | 100G
MOBS >=99% (titration) | Purity: >=99% (titration) | Mol Wt: 223.29 | 115724-21-5 | MFCD00674916 | 100G
Sigma Aldrich Fine Chemicals Biosciences Ethylenediaminetetraacetic acid BioUltra, >=99.0% (KT) | 60-00-4 | MFCD00003541 | 250G
Ethylenediaminetetraacetic acid BioUltra, >=99.0% (KT) | Purity: >=99.0% (KT) | Mol Wt: 292.24 | 60-00-4 | MFCD00003541 | 250G
Sigma Aldrich Fine Chemicals Biosciences Ethylenediaminetetraacetic acid ACS reagent, 99.4-100.6%, powder | 60-00-4 | MFCD00003541 | 1KG
Ethylenediaminetetraacetic acid ACS reagent, 99.4-100.6%, powder | Purity: 99.4-100.6% | Mol Wt: 292.24 | 60-00-4 | MFCD00003541 | 1KG
TARGETMOL CHEMICALS INC P62-mediated mitophagy inducer
Also available in 1 mL, 2 mg, 5 mg, 10 mg, 50 mg, 100 mg, 200 mg and bulk. Please contact Fisher for quotes. P62-mediated mitophagy inducer (PMI) is a regulator of mitophagy. Purity 99.35%
Sigma Aldrich Fine Chemicals Biosciences 5-Cyano-2,3-di-(p-tolyl)tetrazolium chloride >=85% (HPLC) | 90217-02-0 | MFCD00467521 | 50MG
5-Cyano-2,3-di-(p-tolyl)tetrazolium chloride >=85% (HPLC) | Purity: >=85% (HPLC) | Mol Wt: 311.77 | 90217-02-0 | MFCD00467521 | 50MG
Gold Biotechnology Inc Tris (Tris Base) 500 g
GoldBio Tris has the highest purity available on the market. Short for Tris (hydroxymethyl) aminomethane (THAM), Tris is an organic compound often used in buffer solutions such as TAE or TBE for electrophoresis gels. Tris is highly soluble in water and is useful in the pH range 7.0-9.0. It is used in the preparation of Laemmli buffer, one of the most common SDS-PAGE buffers. In addition, Tris can also be used for many custom running and loading buffers, most often with glycine and SDS. The use of single-junction pH electrodes that contain silver should be avoided when determining the pH of a solution containing Tris buffer.
![Research Products International Corp BES [N,N-bis(2-Hydroxyethyl)-2-aminoethanesulfonic acid], 100 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-B32070-100.02.JPG-250.jpg)
Research Products International Corp BES [N,N-bis(2-Hydroxyethyl)-2-aminoethanesulfonic acid], 100 Grams
For use in preparing electrophoresis buffers. pKa (25° C) 7.1. The working pH Range in aqueous solution is 6.4-7.8
![Research Products International Corp HEPES, Free Acid [N-(2-Hydroxyethyl) piperazine N'-(2-ethanesulfonic acid)], 50 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-H75030-50.0.JPG-250.jpg)
Research Products International Corp HEPES, Free Acid [N-(2-Hydroxyethyl) piperazine N'-(2-ethanesulfonic acid)], 50 Grams
HEPES, Free Acid (4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid), is a zwitterionic buffer.
In the life science field, HEPES, Free Acid, is primarily utilized as a buffering agent to maintain a stable pH in various experimental procedures. It is particularly effective in the pH range of 6.8 to 8.2, making it suitable for applications requiring slightly acidic to neutral pH conditions.
HEPES, Free Acid, is commonly used in cell culture media, protein purification, and enzyme assays where a specific pH range needs to be maintained. Its buffering capacity and stability within the desired pH range make it valuable in these applications.
One of the significant advantages of HEPES, Free Acid, is its compatibility with biological systems and enzymes. It exhibits minimal interference with enzymatic activity and other biological processes, ensuring reliable and accurate results.